Bacteriophages as a potential substitute for antibiotics: A comprehensive review.

Over the years, the administration of antibiotics for the purpose of addressing bacterial infections has become increasingly challenging due to the increased prevalence of antimicrobial resistance exhibited by various strains of bacteria. Multidrug-resistant (MDR) bacterial species are rising due to the unavailability of novel antibiotics, leading to higher mortality rates. With these conditions, there is a need for alternatives in which phage therapy has made promising results. Phage-derived endolysins, phage cocktails, and bioengineered phages are effective and have antimicrobial properties against MDR and extensively drug-resistant strains. Despite these, it has been observed that phages can give antimicrobial activity to more than one bacterial species. Thus, phage cocktail against resistant strains provides broad spectrum treatment and magnitude of effectivity, which is many folds higher than antibiotics. Many commercially available endolysins such as Staphefekt SA.100, Exebacase (CF-301), and N-Rephasin®SAL200 are used in biofilm penetration and treating plant diseases. The role of CMP1 phage endolysin in transgenic tomato plants in preventing Clavibacter michiganensis infection and the effectiveness of phage in protecting Atlantic salmon from vibriosis have been reported. Furthermore, phage-derived endolysin therapy, such as TSPphg phage exogenous treatment, can aid in disrupting cell walls, leading to bacterial cell lysis. As animals in aquaculture and slaughterhouses are highly susceptible to bacterial infections, effective phage therapy instead of antibiotics can help treat poultry animals, preserve them, and facilitate disease-free trade. Using bioengineered phages and phage cocktails enhances the effectiveness by providing a broad spectrum of phages and target specificity. Research is currently being conducted on clinical trials to confirm the efficacy of engineered phages and phage cocktails in humans. Although obtaining commercial approval may be time-consuming, it will be beneficial in the postantibiotic era. This review provides an overview of the significance of phage therapy as a potential alternative to antibiotics in combating resistant bacterial strains and its application to various fields and emphasizes the importance of safeguarding and ensuring treatment efficacy.

Examining the factors contributing to a reduction in hardship financing among inpatient households in India.

In India, the rising double burden of diseases and the low fiscal capacity of the government forces people to resort to hardship financing. This study aimed to examine the factors contributing to the reduction in hardship financing among inpatient households in India. The study relies on two rounds of National Sample Surveys with a sample of 34,478 households from the 71st round (2014) and 56,681 households from the 75th round (2018). We employed multivariable logistic regression and multivariate decomposition analyses to explore the factors associated with hardship financing in Indian households with hospitalized member(s) and assess the contributing factors to the reduction in hardship financing between 2014 and 2018. Notably, though hardship financing for inpatient households has decreased between 2014 and 2018, households with catastrophic health expenditure (CHE) had higher odds of hardship financing than those without CHE. While factors such as CHE, prolonged hospitalization, and private hospitals had impoverishing effects on hardship financing in 2014 and 2018, the decomposition model showed the potential of CHE (32%), length of hospitalization (32%), and private hospitals (24%) to slow down this negative impact over time. The findings showed the potential for further improvements in financial health protection for inpatient care over time, and underscore the need for continuing efforts to strengthen the implementation of public programs and schemes in India such as Ayushman Bharat Pradhan Mantri Jan Arogya Yojana (PMJAY).

Experimental investigation into mechanical, thermal, and shape memory behavior of thermoresponsive PU/MXene shape memory polymer nanocomposite.

This research presents an experimental investigation into the mechanical, thermal, and shape memory behavior of a thermos-responsive polyurethane (PU) reinforced with 0-1.0 wt % of MXene (Ti3C2Tx) nanofiller. The PU/MXene nanocomposites were fabricated using sonication and injection molding route. The 0.5 wt % PU/MXene nanocomposite showed the optimum mechanical properties i.e. tensile modulus, tensile strength, and hardness value, which are improved by 22, 281, and 19 %, respectively, compared to pure PU. The improvement is observed in melting temperature (Tm), the heat of melting (hm), crystallization temperature (Tc), and the heat of crystallization (hc) results. The percentage of crystallinity revealed enhancements of 6 %, 18 %, 24 %, and 34 % for 0.1, 0.2, 0.3, and 0.5 wt % PU/MXene samples respectively compared to pure PU. The findings from the shape recovery experiments demonstrated that the inclusion of MXene has no impact on both the shape fixity and shape recovery performance. The PU/MXene nanocomposite with improved mechanical and thermal properties can find potential applications in robotics actuators, medical devices, sensors, etc.

Pigmented Villonodular Synovitis of Ankle Joint - A Case Report.

Introduction: Pigmented villonodular synovitis (PVNS)is a very uncommon lesion that involves the synovium of joints, tendon sheaths, or bursae. Approximately 2.5% of cases occur around the ankle. PVNS was earlier considered to be a synovial malignancy. Complete surgical excision is curative if bony destruction is not there. Radiotherapy is rarely helpful for residual lesions. Case Report: We share a case report on PVNS of ankle in a 22-years old male patient. Clinical diagnosis was confirmed with the help of magnetic resonance imaging and histopathology. A surgical excision with synovectomy was carried out. There were no signs of recurrence in 1.5 years follow-up. Conclusion: Complete surgical excision with radical synovectomy is crucial for the treatment of PVNS in rare locations like the ankle joint.

Green synthesis of N,S-doped carbon dots for tartrazine detection and their antibacterial activities.

A simple, green and low-cost method was developed for the synthesis of highly fluorescent N,S-doped carbon dots (N,S-CDs) via the hydrothermal treatment of Gandha Prasarini (GP) leaves as a natural source of carbon, nitrogen and sulfur. The as-prepared N,S-CDs exhibited excitation-dependent green fluorescence emission (λex = 450 nm, λem = 525 nm) with excellent stability, and were used as a fluorescent probe for the selective detection of tartrazine with a limit of detection of 0.18 µM. The fluorescence quenching of N,S-CDs was due to the inner filter effect. The developed method has been employed for the determination of tartrazine in honey and soft drinks with satisfactory recovery ranging from 92 to 110.2%. In addition, the antibacterial activity of the N,S-CDs was explored against both Gram-negative bacteria, Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), and Gram-positive bacteria, Staphylococcus aureus (S. aureus). The antibacterial mechanism of the N,S-CDs was investigated. The results indicated that the antibacterial activity was due to the membrane damage of the bacteria by the N,S-CDs. Besides, the N,S-CDs showed negligible lytic effects on human erythrocytes. These findings will inspire further exploitation of CD-based nano-bactericides in biomedical applications.

Isolated Osteomyelitis of the Cuboid Treated with One-Stage Debridement and Bone Grafting - A Case Series and Literature Review.

Introduction: Isolated osteomyelitis of the cuboid is a rare entity with very few case reports worldwide. A variety of treatment methods are described for these lesions, both single-stage and two staged and ranges from simple curettage to bone grafting and arthrodesis. Case Report: We present a series of two cases of chronic osteomyelitis in young adults due to puncture wound over the lateral foot. Both patients presented with purulent discharge from sinus over lateral foot. There was no involvement of adjoining bones in them. Culture yielded Staphylococcus aureus and Pseudomonas aeruginosa. Both patients were treated with adequate curetting, saucerization with cancellous bone grafting in one of the cases. Both wounds healed uneventfully with good ankle and hindfoot function. Conclusion: The cuboid is a rare site of chronic osteomyelitis due to punture wounds with foreign bodies, especially in rural population. With meticulous curettage and bone grafting, the infection can be eradicated reliably and usually with good residual function.

Evaluation of tensile properties of spherical shaped SiC inclusions inside recycled HDPE matrix using FEM based representative volume element approach.

In the current study, a FEM-based representative volume element (RVE) technique is used to evaluate the elastic modulus of recycled high-density polyethylene (rHDPE) filled spherical-shaped shaped silicon carbide (SiC). In the ANSYS 2019, the material designer (MD) module is used to generate a 3D RVE of 500 × 500 × 500 µm cuboid, with randomly dispersed spherical SiC particles (i.e., 10, 15, 20, and 30% volume fractions) inside rHDPE. The Young's modulus values extracted from the RVE technique at various volume % are substantially nearer to experimental data than other micromechanical models. The tensile performance of the composite is simulated, and it was noted that the maximum equivalent stress of 4.1133 MPa for rHDPE/30% SiC composite, which is decreased to 13.8, 7.8 and 6.8% for rHDPE/10% SiC, rHDPE/15% SiC and rHDPE/20% SiC composite respectively. The results are astounding for immediate application in the relevant field of interest.

Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles.

Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimicrobial potential, low toxicity to humans, high thermal stability, biocompatibility, and low cost of production. However, their successful transformation into sustainable drugs is limited due to their low membrane permeability, which reduces their bioavailability in target cells. Herein we propose Cerium-doped magnesium oxide nanoparticles (MgOCeNPs) as a powerful solution to above mentioned limitations and are compared with MgO NPs for their membrane permeability and antimicrobial activity. Both pure and Ce-doped were characterized by various spectroscopic and microscopic techniques, in which an X-ray diffraction (XRD) examination reveals the lattice patterns for doped nanoparticles. Furthermore, Atomic Force Microscopy (AFM) revealed the three-dimensional (3D) structure and height of the nanoparticle. The crystal structure (FCC) of MgO did not change with Ce doping. However, microstructural properties like lattice parameter, crystallite size and biological activity of MgO significantly changed with Ce doping. In order to evaluate the antimicrobial potential of MgOCeNPs in comparison to MgO NPs and to understand the underlying mechanisms, the antibacterial activity was investigated against human pathogenic bacteria E. coli and P. aeruginosa, and antifungal activity against THY-1, a fungal strain. MgOCeNPs were studied by several methods, which resulted in a strong antibacterial and antifungal activity in the form of an elevated zone of inhibition, reduced growth curve, lower minimum inhibitory concentration (MIC80) and enhanced cytotoxicity in both bacterial and fungal strain as compared to MgO nanoparticles. The study of the growth curve showed early and prolonged stationary phase and early decline log phase. Both bacterial and fungal strains showed dose-dependent cytotoxicity with enhancement in intracellular reactive oxygen species (ROS) generation and formation of pores in the membrane when interacting with egg-phosphatidylcholine model Large Unilamellar Vesicles (LUVs). The proposed mechanism of MgOCeNPs toxicity evidently is membranolytic activity and induction of ROS production, which may cause oxidative stress-mediated cytotoxicity. These results confirmed that MgOCeNPs are a novel and very potent antimicrobial agent with a great promise of controlling and treating other microbes.

Illnesses and hardship financing in India: an evaluation of inpatient and outpatient cases, 2014-18.

BACKGROUND: Progress towards universal health coverage requires strengthening the country's health system. In developing countries, the increasing disease burden puts a lot of stress on scarce household finances. However, this burden is not the same for everyone. The economic burden varies across the disease groups and care levels. Government intervention is vital in formulating policies in addressing financial distress at the household level. In India, even when outpatient care forms a significant proportion of out-of-pocket expenditure, government schemes focus on reducing household expenditure on inpatient care alone. Thus, people resort to hardship financing practices like informal borrowing or selling of assets in the event of health shocks. In this context, the present study aims to identify the disease(s) that correlates with maximum hardship financing for outpatients and inpatients and to understand the change in hardship financing over time. METHODS: We used two waves of National Sample Survey Organisation's data on social consumption on health- the 71st and the 75th rounds. Descriptive statistics are reported, and logistic regression is carried out to explain the adjusted impact of illness on hardship financing. Pooled logistic regression of the two rounds is estimated for inpatients and outpatients. Marginal effects are reported to study the changes in hardship financing over time. RESULTS: The results suggest that cancer had the maximum likelihood of causing hardship financing in India for both inpatients (Odds ratio 2.41; 95% Confidence Interval (CI): 2.03 - 2.86 (71st round), 2.54; 95% CI: 2.21 - 2.93 (75th round)) and outpatients (Odds ratio 6.11; 95% CI: 2.95 - 12.64 (71st round), 3.07; 95% CI: 2.14 - 4.40 (75th round)). In 2018, for outpatients, the hardship financing for health care needs was higher at public health facilities, compared to private health facilities (Odds ratio 0.72; 95% CI: 0.62 - 0.83 (75th round). The marginal effects model of pooled cross-section analysis reveals that from 2014 to 2018, the hardship financing had decreased for inpatients (Odds ratio 0.747; 95% CI:0.80 - -0.70), whereas it had increased for outpatients (Odds ratio 0.0126; 95% CI: 0.01 - 0.02). Our results also show that the likelihood of resorting to hardship financing for illness among women was lesser than that of men. CONCLUSION: Government intervention is quintessential to decrease the hardship financing caused by cancer. The intra-household inequalities play an important role in explaining their hardship financing strategies. We suggest the need for more financial risk protection for outpatient care to address hardship financing.

Zymography assisted quick purification, characterization and inhibition analysis of K. pneumoniae alkaline phosphatase by mercury and thiohydroxyal compounds.

In-gel hydrolysis of para-nitrophenyl phosphate (p-NPP) to yellow colored para-nitrophenol was used to locate precisely the K. pneumoniae alkaline phosphatase (Kp-ALKP) on 7% native PAGE. Subsequent removal of the yellow-stained band and electroelution yielded a 54 kDa, Kp-ALKP with Km, Vmax and kcat values of (0.7 ± 0.02) mM, (80 ± 4.5) µmol min-1 and (39.2 ± 2.2) × 104 s-1 respectively for p-NPP. Kp-ALKP was optimally active at 70 °C and pH 7.2 that was activated by Mg2+, Ca2+, Co2+ and inhibited by EDTA, PO4, Pb2+, Cu2+ and Hg2+. The enzyme was trypsin resistant and retained 75% activity in presence of 10 mM PO4 and 65% activity at 3 mM Hg2+ showing it's PO43- irrepressibility and Hg2+-tolerance. Molecular dynamics simulation revealed increased structural stability of Kp-ALKP at 70 °C that accounts for it's optimal temperature. Zymography revealed that both DTT and ß-mercaptoethanol induced activity loss accompanied by mobility retardation of Kp-ALKP on 7% native PAGE. These results and in Silico analysis shows that both DTT and ßME reduce the C308-C358 disulfide bond, leading to an open conformation of the enzyme. However, Hg2+ had negligible effect on the in-gel mobility of Kp-ALKP indicating it's plausible non-covalent interaction with surface-accessible amino-acids without significant conformational change. For the first time our study reveals the zymography as an easy, inexpensive and convenient tool for quick purification, characterization and conformational analysis of K. pneumoniae alkaline phosphatase.

Experimental Investigation of Electro-Mechanical Behavior of Silver-Coated Teflon Fabric-Reinforced Nafion Ionic Polymer Metal Composite with Carbon Nanotubes and Graphene Nanoparticles.

Ionic Polymer Metal Composites (IPMCs) are in high demand owing to the ongoing advancements in technology for various applications. New fabrication techniques and a quick retort towards the applied load are the significant reasons for considering IPMCs in smart devices. Here, a Teflon fabric-reinforced Nafion (TFRN) membrane is used to create an IPMC. The materials employed as electrodes are silver and nanofillers. The basement membrane, Nafion 438 (N-438), is sandwiched between the electrodes using a chemical decomposition technique. Subsequently, the electromechanical properties (actuation) of the membrane are tested. The micro and molecular structure of the IPMC membrane coated with Silver (Ag), Ag-Carbon nanotubes (CNTs), and Ag-Graphene nanoparticles samples are examined with the help of SEM and X-ray diffraction (XRD). The membrane scratch test is carried out to evaluate the abrasion and wear resistance of the membrane. The lowest coefficient of friction is shown by N438 + Ag + Graphene (0.05), which increased by 300% when compared to a pure N438 membrane. The hydration and tip deflection test were also performed to understand the water uptake percentage. At 90 °C, the highest water uptake was observed for N438 + Ag + Graphene (0.05), which decreased by 60, 42, 23, 14 and 26% when compared to N438, N438 + Ag, N438 + Ag + CNT (0.01), N438 + Ag + CNT (0.05) and N438 + Ag + Graphene (0.01), respectively. A proportional relationship between hydration level and tip deflection is observed and the highest bending performance is observed for the N438 + Ag + Graphene (0.05) membrane.

A Comprehensive Review of Self-Healing Polymer, Metal, and Ceramic Matrix Composites and Their Modeling Aspects for Aerospace Applications.

Composites can be divided into three groups based on their matrix materials, namely polymer, metal and ceramic. Composite materials fail due to micro cracks. Repairing is complex and almost impossible if cracks appear on the surface and interior, which minimizes reliability and material life. In order to save the material from failure and prolong its lifetime without compromising mechanical properties, self-healing is one of the emerging and best techniques. The studies to address the advantages and challenges of self-healing properties of different matrix materials are very limited; however, this review addresses all three different groups of composites. Self-healing composites are fabricated to heal cracks, prevent any obstructed failure, and improve the lifetime of structures. They can self-diagnose their structure after being affected by external forces and repair damages and cracks to a certain degree. This review aims to provide information on the recent developments and prospects of self-healing composites and their applications in various fields such as aerospace, automobiles etc. Fabrication and characterization techniques as well as intrinsic and extrinsic self-healing techniques are discussed based on the latest achievements, including microcapsule embedment, fibers embedment, and vascular networks self-healing.

Harnessing the Role of Bacterial Plasma Membrane Modifications for the Development of Sustainable Membranotropic Phytotherapeutics.

Membrane-targeted molecules such as cationic antimicrobial peptides (CAMPs) are amongst the most advanced group of antibiotics used against drug-resistant bacteria due to their conserved and accessible targets. However, multi-drug-resistant bacteria alter their plasma membrane (PM) lipids, such as lipopolysaccharides (LPS) and phospholipids (PLs), to evade membrane-targeted antibiotics. Investigations reveal that in addition to LPS, the varying composition and spatiotemporal organization of PLs in the bacterial PM are currently being explored as novel drug targets. Additionally, PM proteins such as Mla complex, MPRF, Lpts, lipid II flippase, PL synthases, and PL flippases that maintain PM integrity are the most sought-after targets for development of new-generation drugs. However, most of their structural details and mechanism of action remains elusive. Exploration of the role of bacterial membrane lipidome and proteome in addition to their organization is the key to developing novel membrane-targeted antibiotics. In addition, membranotropic phytochemicals and their synthetic derivatives have gained attractiveness as popular herbal alternatives against bacterial multi-drug resistance. This review provides the current understanding on the role of bacterial PM components on multidrug resistance and their targeting with membranotropic phytochemicals.

A Brief Review on Advanced Sandwich Structures with Customized Design Core and Composite Face Sheet.

Sandwich structures are a class of multifunctional high-performance structural composites that have the advantages of being lightweight, of a high strength-to-weight ratio, and of high specific energy absorption capabilities. The creative design of the core along with the apposite material selection for the fabrication of the face sheet and core are the two prerequisites with encouraging areas for further expedition towards the fabrication of advanced composite sandwich structures. The current review work focused on different types of core designs, such as truss, foam, corrugated, honeycomb, derivative, hybrid, hollow, hierarchical, gradient, folded, and smart core along with different composite materials accessible for face sheet fabrication, including fiber-reinforced composite, metal matrix composite, and polymer matrix composite are considered. The joining method plays a major role for the performance evolution of sandwich structures, which were also investigated. Further discussions are aligned to address major challenges in the fabrication of sandwich structures and further enlighten the future direction of the advanced composite sandwich structure. Finally, the work is summarized with a brief conclusion. This review article provides wider guidelines for researchers in designing and manufacturing next-generation lightweight multilayer core sandwich structures.

Phospholipid Scramblases: Role in Cancer Progression and Anticancer Therapeutics.

Phospholipid scramblases (PLSCRs) that catalyze rapid mixing of plasma membrane lipids result in surface exposure of phosphatidyl serine (PS), a lipid normally residing to the inner plasma membrane leaflet. PS exposure provides a chemotactic eat-me signal for phagocytes resulting in non-inflammatory clearance of apoptotic cells by efferocytosis. However, metastatic tumor cells escape efferocytosis through alteration of tumor microenvironment and apoptotic signaling. Tumor cells exhibit altered membrane features, high constitutive PS exposure, low drug permeability and increased multidrug resistance through clonal evolution. PLSCRs are transcriptionally up-regulated in tumor cells leading to plasma membrane remodeling and aberrant PS exposure on cell surface. In addition, PLSCRs interact with multiple cellular components to modulate cancer progression and survival. While PLSCRs and PS exposed on tumor cells are novel drug targets, many exogenous molecules that catalyze lipid scrambling on tumor plasma membrane are potent anticancer therapeutic molecules. In this review, we provide a comprehensive analysis of scramblase mediated signaling events, membrane alteration specific to tumor development and possible therapeutic implications of scramblases and PS exposure.

Exploring the possible sources of fiscal space for health in India: insights from political regimes.

BACKGROUND: Rising healthcare costs and poor access to health services have become a significant concern for policy-makers; therefore, efforts must be made to generate fiscal space through alternative revenue measures in resource-poor economies. This study attempts to identify possible sources of fiscal space for health in India across political regimes. METHODS: The study followed a descriptive approach to examine the political commitment towards health sector development by estimating the trend of growth in fiscal space indicators over the political regimes from 1998-1999 to 2021-2022 using a dummy variable regression model. RESULTS: We found four possible sources of fiscal space for health, which include (1) raising domestic revenue mobilization, (2) generating alternative revenue collection mechanisms, (3) prioritizing health through expenditure management and (4) effective utilization of central transfer. Fiscal space measures such as goods and services tax reform, collection of health-specific tax, higher excise duty on tobacco products, cooking gas subsidies to poor people, tax administration reform and direct beneficiary transfer of health services could be alternative revenue mobilization channels for fiscal space for health. CONCLUSION: The study reveals that the central government has a political commitment to generating revenue through various fiscal policy reforms. Health has been prioritized over the period, but there is less evidence of health-related political commitment for an increased share of health expenditure to total budgetary allocation. During the last 2 years, however, the health budget has been prioritized due to the COVID-19 pandemic crisis despite slower economic growth in India. This study will be a policy document for fiscal space analysis from a political-economic perspective, and the role of the ministry of finance can be assessed through administrative data and documents.

The Impact of COVID-19 on the Household Economy of India.

COVID-19 has disrupted the Indian economy. Government-enforced lockdown to restrict the spread of infection has impacted the household economy in particular. We combine aggregates from national income accounts and estimates from the microdata of a labour force survey covering more than 0.1 million households and 0.4 million individuals. The aggregate daily loss to households is USD 2.42 billion. While loss to earnings accounts for 72% of the total, the rest 28% is wage loss. Service-based activities account for two thirds of wage loss, and natural resource-based activities are responsible for most of the earning loss. The dominance of informal job contracts and job switching in labour markets intensifies this, with the most vulnerable group consisting of 57.8 million in casual engagement, who have a high degree of transition from one stream of employment to another on a daily basis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s41027-021-00352-8.

An Ensemble-Based Scalable Approach for Intrusion Detection Using Big Data Framework.

In this study, we set up a scalable framework for large-scale data processing and analytics using the big data framework. The popular classification methods are implemented, tuned, and evaluated by using intrusion datasets. The objective is to select the best classifier after optimizing the hyper-parameters. We observed that the decision tree (DT) approach outperforms compared with other methods in terms of classification accuracy, fast training time, and improved average prediction rate. Therefore, it is selected as a base classifier in our proposed ensemble approach to study class imbalance. As the intrusion datasets are imbalanced, most of the classification techniques are biased toward the majority class. The misclassification rate is more in the case of the minority class. An ensemble-based method is proposed by using K-Means, RUSBoost, and DT approaches to mitigate the class imbalance problem; empirically investigate the impact of class imbalance on classification approaches' performance; and compare the result by using popular performance metrics such as Balanced Accuracy, Matthews Correlation Coefficient, and F-Measure, which are more suitable for the assessment of imbalanced datasets.

Lipid Flip-Flop-Inducing Antimicrobial Phytochemicals from Gymnema sylvestre are Bacterial Membrane Permeability Enhancers.

An amphiphilic phytochemical fraction isolated from methanol extract of Gymnema sylvestre leaf powder contained six terpenoids, two flavonoids, and one alkaloid that induced rapid flip-flop of fluorescent phospholipid analog in the phosphatidyl choline bilayer. Lipid-flipping activity of the methanol-extracted fraction of G. sylvestre (MEFGS) was dose-dependent and time-dependent with a rate constant k = (12.09 ± 0.94) mg-1 min-1 that was saturable at (40 ± 1) % flipping of the fluorescent lipid analogue. Interactions of MEFGS phytochemicals with large unilamelar vesicles led to time-dependent change in their rounded morphology into irregular shapes, indicating their membrane-destabilizing activity. MEFGS exhibited antibacterial activity on Escherichia coli (MTCC-118), Staphylococcus aureus (MTCC-212), and Pseudomonas aeruginosa (MTCC-1035) with IC50 values 0.5, 0.35, and 0.1 mg/mL, respectively. Phytochemicals in MEFGS increased membrane permeabilization in all three bacteria, as indicated by 23, 17, and 17% increase in the uptake of crystal violet, respectively. MEFGS enhanced membrane damage, resulting in a 3-5 fold increase in leakage of cytosolic ions, 0.5-2 fold increase in leakage of PO4 -, and 15-20% increase in loss of cellular proteins. MEFGS synergistically increased the efficacy of curcumin, amoxillin, ampicillin, and cefotaxime on S. aureus probably by enhancing their permeability into the bacterium. For the first time, our study reveals that phytochemicals from G. sylvestre enhance the permeability of the bacterial plasma membrane by facilitating flip-flop of membrane lipids. Lipid-flipping phytochemicals from G. sylvestre can be used as adjuvant therapeutics to enhance the efficacy of antibacterials by increasing their bioavailability in the target bacteria.

Electroformation of liposomes and phytosomes using copper electrode.

A novel method for electroformation of liposomes and phytosomes using copper electrode is described. Liposomes made at 2 V and 10 Hz AC field from L-α-egg-phosphatidylcholine (egg-PC), K. pneumoniae phosphatidylethanolamine, K. pneumoniae polar lipids and E. coli polar lipids on copper electrode were (777.9 ± 118.4), (370.2 ± 100.5), (825.3 ± 21.54), and (281.3 ± 42.3) nm in diameter, respectively. Giant vesicles were formed at 30 V and 10 Hz AC field from polar lipids of K. pneumoniae and E. coli were (106 ± 29.7) and (86 ± 24.3) µm in diameter, respectively. All liposomes were unilamellar as indicated by their unilamellar indices of 50 ± 2, had surface charge comparable to vesicles made from lipid(s) with similar composition and exhibited only 1-2 mol% of oxidized lipids. Cu concentration in the liposomal samples was <1.5 ppm for large unilamellar vesicles (LUVs) and ˂5 ppm for giant unilamellar vesicles (GUVs). The vesicles were stable for >15 d without loss of their size, charge, or unilamellarity. The method was successfully applied to prepare phytosomes from egg-PC and a phytochemical fraction of Dimorphocalyx glabellus, a medicinal plant with anti-diuretic properties. Phytosomes formed were 1000-1500 nm in diameter and exhibited altered fluorescence and absorbance properties compared to the unencapsulated phytochemical. Phytosomes with phytochemical: egg-PC ratio from 0.15 to 1.5 had encapsulation efficiency ranging 90-30%, respectively, and was stable for 1 month. Our method is easy, inexpensive and convenient that will prove to be useful for preparation of liposomes and phytosomes.